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KMID : 0043320070300010109
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Archives of Pharmacal Research
2007 Volume.30 No. 1 p.109 ~ p.113
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Inhibitory Effects of (1R,9S)-β-Hydrastine on Calcium Transport in PC12 Cells
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Yin Shou Yu
Jin Chun Mei
Yang Yoo-Jung
Lim Sung-Cil
Lee Chong-Kil
Hwang Bang-Yeon
Ro Jai-Seup
Lee Myung-Koo
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Abstract
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(1R,9S)-β-Hydrastine (BHS), at 100 μM, has been shown to mainly reduce the K+-induced dopamine release and Ca2+ influx by blocking the L-type Ca2+ channel and inhibit the caffeine activated store-operated Ca2+ channels, but not those activated by thapsigargin, in PC12 cells. In this study, the effects of BHS on Ca2+ transport from Ca2+ stores in the absence of external Ca2+ were investigated in PC12 cells. BHS decreased the basal intracellular Ca2+ concentration ([Ca2+]i) in the absence of external Ca2+ in PC12 cells. In the absence of external Ca2+, pretreating PC12 cells with 100 μM BHS reduced the rapid increase in the [Ca2+]i elicited by 20mM caffeine, but not that by 1 μM thapsigargin. In addition, BHS inhibited the increase in the [Ca2+]i elicited by restoration of 2 mM CaCl2 after the Ca2+ stores had been depleted by 20 mM caffeine, but not those depleted by 1 μM thapsigargin, in the absence of external Ca2+. These results suggested that BHS mainly inhibited Ca2+ leakage from the Ca2+ stores and the caffeine-stimulated release of Ca2+ from the caffeine-sensitive Ca2+ stores in PC12 cells.
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KEYWORD
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(1R, 9S)-β-Hydrastine, Caffeine-stimulated Ca2+ release, Store-operated Ca2+ channels, PC12 cells
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